ITEM #: 16680397
Inflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is a true for horses as it is for humans. Both experience immunological diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines...
ITEM #: 16680397
Inflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is a true for horses as it is for humans. Both experience immunological diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines and chemokine in horses has been limited primarily to ELISAs. However, researchers will now the ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX MAP Equine Cytokine/Chemokine Panel, based on the Luminex xMAP technology.
EMD Millipore’s MILLIPLEX MAP Equine Cytokine Magnetic Bead Panel is a 23-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: Eotaxin/CCL11, FGF-2. Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα. MILLIPLEX MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX MAP panel and kit includes: - Quality controls (QCs) provided to qualify assay performance - Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency - Optimized serum matrix to mimic native analyte environment - Detection antibody cocktails designed to yield consistent analyte profiles within panel EMD Millipore’s MILLIPLEX MAP Equine Cytokine Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Panel Type: MAGNETIC Cytokines/Chemokines
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
• This is an overnight (16-18 hours at 2-8 °C) or two-hour (20-25°C) incubation assay; although an overnight incubation is recommended. • A maximum of 25 µL per well of neat serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. • This kit may be used for the analysis of all or any combination of the Equine Cytokine/Chemokine Panel analytes in serum, plasma or tissue/cell/tumor culture supernatant.
Research Category Inflammation & Immunology
Used to detect/quantify: Eotaxin/CCL11 FGF-2 Fractalkine/CX3CL1 G-CSF GM-CSF GRO/KC IFN-γ IL-1α IL-1β IL-2 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 (p70) IL-13 IL-17A IL-18 IP-10 MCP-1 RANTES TNF-α
Design your multiplex kit by choosing available analytes within this panel.
96 well plate
Recommended storage for kit components is 2 - 8°C.
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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