ITEM #: 13103389VenorGeM Advance contains PCR reaction tubes pre-coated with all PCR reagents to reduce the total assay time without need to prepare aliquots of a master mi x . For additional convenience the gel loading buffer and dye are already included in the reaction buffer. After thermal cycling the PCR can be loaded directly on the agarose gel.
Type of PCR
For conventional, endpoint PCR
Scope of Application
Applicable in research for direct testing of cell cultures and cell culture-derived biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Kit Components
- Primer sets, nucleotides, polymerase and internal control DNA, lyophilized, filled in 0.2 ml PCR reaction tubes
- Rehydration buffer including gel loading buffer and running dye
- Positive controls, lyophilized, filled in 0.2 ml PCR reaction tubes
- PCR grade water
Result evaluation
Gel electrophoresis at endpoint of PCR
Required Consumables
Agarose and DNA-specific dye for gel staining
- Required lab devices
- Regular block-heated PCR cycler
- Agarose gel electrophoresis and gel documentation system
- Pipetting equipment
- Tube centrifuge
Shelf Life and Storage
Kit components are maintainable at +2 to +8°C for at least 6 months. After rehydratisation of the individual PCR tubes the reagents must be used within 1 hour.
EP 2.6.7 compliance
No
VenorGeM Advance, 240 Tests
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